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KMID : 1098419970050010036
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Korean Journal of Medicinal Crop Science
1997 Volume.5 No. 1 p.36 ~ p.42
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Effects of Colchicine and EMS Dropping to Apical Meristem on Induced Mutation and Plant Protein Pattern in Wasabia japonica Matsum
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Park Kie-In
Eun Jong-Seon
Choi Sun-Yong
Kwon Sung-Whan
Lee Kang-Soo
So Sang-Sup
Youn Myung-Ja
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Abstract
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This study was investigated on the effects of colchicine and EMS. induced mutation, plant on the germination and polypeptide pattern and peroxidase activity monitored by two dimensional gel analysis in Wasabia japonica Matsum (wasabi). Germination rate of Muju was higher than that of Ulrung-Do and optimum concentration for germination was appeared 100 ppm GA3 containing with 10 ppm BAP in these cultivars. Survived plants rate of Muju was higher than that of Ulrung-Do after colchicine and EMS treatment. Peroxidase activity and plant height were decreased by mutagen treatments, while incresed in root and stem thickness. The number of protein spots and pattern showed difference between Muju and Ulrung-Do . The plants treated mutagen increased polypeptide spots, especially EMS treatment showed more different polypeptide pattern compared to control.
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